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The early S-phase focal replication pattern does not result from differential access of DNaseI. Each column reflects a single cell stained for DAPI (upper panels), BrdU incorporation (middle panels), or TUNEL (lower panels, Intergen) at the indicated DNaseI concentration. Cells were treated with DNaseI (Sigma) and stained with an anti-BrdU antibody (Becton Dickinson). After secondary antibody application, cells were processed for TUNEL staining.
