Figure 9.
pRB family members localize to early S-phase sites of DNA synthesis. Synchronized cells were pulsed with BrdU in either early S-phase (upper panels) or mid-to-late S-phase (lower panels). Sites of DNA synthesis were determined in by indirect immunofluorescence (red) and compared to the localization pattern of pRB (green; A) or p130 (green; B). Overlapping signals appear yellow. (C) The localization patterns of replication proteins were compared to those of pRB family members in cells arrested in G1 by contact inhibition. Left panel: p130 (red), p150 (CAF-1; green). Right panel: p130 (green), Lamin A/C (red).