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. 2011 Aug 29;10:104. doi: 10.1186/1476-4598-10-104

Figure 2.

Figure 2

Apigenin induces apoptosis and downregulates the expression of antiapoptotic proteins in MM cells. (A) U266 and RPMI 8226 cells were treated with apigenin for 24 h, apoptotic cell death was detected by staining cells with the Annexin V-FLUOS kit and analyzed by flow cytometry. (B) U266 and RPMI 8226 cells were treated with increasing doses of apigenin for 24 h, and the antiapoptotic proteins were detected by western blot analysis using the indicated antibodies; β-actin was used as a loading control.