Table 2.
Nucleotide positions significantly enriched in OBI dataset by univariable analysis
| Nucleotide positions* | Adjusted p-value | Significant nt changes in OBI vs. non-OBI | Amino acid change in affected open reading frames | Affected HBV protein** | |||
|---|---|---|---|---|---|---|---|
| polymerase | surface | core | |||||
| pre-S2 | S | ||||||
| 78 | 0.0144 | G → A | no change | Gly → Glu | L, M | ||
| 233 | 0.0367 | A → G | His → Arg (RT domain) | Thr → Ala | P, L, M, S | ||
| 418 | 0.0367 | G → T | Ala → Ser (RT domain) | no change | P | ||
| 2240 *** | 0.0097 | A → G | Thr → Val (T helper epitope) *** | C | |||
| 2241 *** | 0.0144 | C → T | Thr → Val (T helper epitope) *** | C | |||
| 2435 | 0.0367 | C → A | no change | Gln → Lys (Arg-rich C-terminus) | C | ||
| 2485 | 0.0367 | A → G | Asn → Ser (TP domain) | P | |||
* positions in the well-annotated reference HBV sequence Acc.No. AM282986
** L, M, S: Large, Middle and Small S, respectively; P: Polymerase; C: Core
*** nucleotides 2240 and 2241 co-variated in all observed cases, substituting ACT with GTT or ACA with GTA codons.