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. 1999 Oct 1;13(19):2484–2489. doi: 10.1101/gad.13.19.2484

Figure 3.

Figure 3

Figure 3

Taf40 is generally required for in vivo transcription by Pol II. (A) S1 nuclease protection assay of mRNAs in the taf40-3100 mutant strain. RNA was isolated from wild-type or mutant strains incubated at 37°C for the indicated amount of time. Probes for the RPS4, DED1, HTA2, and ACT1 genes were used to monitor levels of those transcripts. A probe for Pol III-transcribed tRNAW was used as an internal control for RNA normalization. (B) Total poly(A)+ levels in strains carrying either the indicated wild-type or the mutant TAF40 alleles. Total RNAs from wild-type or the indicated mutant TAF strains were isolated as described in A and slot-blotted to a membrane. Poly(A)+ levels were assayed by hybridization with a radioactive oligo(dT) probe.