Figure 4.
Effect of Taf40 temperature inactivation on the levels of TFIID and SAGA components. Aliquots of wild-type or taf40-3100 mutant strains were taken after the indicated time at 30°C or 37°C. Total protein was blotted and probed with antibodies to the indicated proteins. In the case of Taf40 and Taf61/68 a background band was seen nearby; the actual TAF band is indicated by an asterisk. Other taf40 temperature-sensitive alleles had similar effects on protein levels at the nonpermissive temperature (data not shown).