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. 1999 Oct 1;13(19):2484–2489. doi: 10.1101/gad.13.19.2484

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Copyright © 1999, Cold Spring Harbor Laboratory Press

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TBP overexpression does not restore transcription to a taf40-3100 mutant strain. (A) Samples of a wild-type (TAF40) and mutant strain (taf40-3100) carrying either vector or an epitope-tagged TBP overexpression construct (PGK1pr–TBP) were taken after the indicated time at 37°C. Total protein was blotted and probed with antibodies to TBP. The positions of the endogenous (TBP) and overexpressed, tagged TBP (HA₃–TBP) are indicated. (B) Total RNA was isolated from the cultures used in A and analyzed for total poly(A)⁺ RNA as described in Fig. 3.

TBP overexpression does not restore transcription to a taf40-3100 mutant strain. (A) Samples of a wild-type (TAF40) and mutant strain (taf40-3100) carrying either vector or an epitope-tagged TBP overexpression construct (PGK1pr–TBP) were taken after the indicated time at 37°C. Total protein was blotted and probed with antibodies to TBP. The positions of the endogenous (TBP) and overexpressed, tagged TBP (HA₃–TBP) are indicated. (B) Total RNA was isolated from the cultures used in A and analyzed for total poly(A)⁺ RNA as described in Fig. 3.