Cos2 inhibits the activity of uncleavable forms of Ci. Wing discs shown in this and following figures were derived from late third-instar larvae and are oriented with anterior toward the left and ventral up. Wing discs in A,B,E–H were stained with anti-βgal antibody to visualize ptc-lacZ expression (green). Wing discs in C and D were stained with anti-Ci antibody (2A1) that recognizes full-length Ci (red). The genotypes for the wing discs are as follows: (A,C) wild type; (B,D) MS1096, UAS-Cos2; (E) MS1096, UAS-CiU; (F) MS1096, UAS-CiU, UAS-Cos2; (G) MS1096, UAS-Ci-3P; (H) MS1096, UAS-Ci-3P, UAS-Cos2.