Figure 3.

The meiotic DNA replication checkpoint arrests cells in prophase. The pat1^ts/pat1^ts (A) and pat1^ts/pat1^ts rad3/rad3 cells (B) were induced to undergo meiosis as in Fig. 2 for 6 hr at 34°C in HU, processed for immunofluorescence and stained with anti-TAT1 and anti-Sad1 antibodies. Typical examples of images of a field of the cells are shown. (Top) Position and morphology of chromatin DNA stained with DAPI; (middle) anti-tubulin staining probed with anti-TAT1 antibody; (bottom) anti-Sad1 staining for a component of the SPB.