Table 1.
Viability of rescued erbB2 mutant embryos
| Genotype of parents |
Nkx2.5erbB2/+; erbB2+/− × Nkx2.5+/+; erbB2+/−
|
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Genotype of litter |
Nkx2.5erbB2/+
|
Nkx2.5+/+
|
||||||||||||
|
erbB2+/+
|
erbB2+/−
|
erbB2−/−
|
erbB2+/+
|
erbB2+/−
|
erbB2−/−
|
|||||||||
| Expected ratio
|
|
12.5%
|
|
25%
|
|
12.5%
|
|
12.5%
|
|
25%
|
|
12.5%
|
|
|
| E10.5 | 3 | 10% | 8 | 27% | 4 | 13% | 4 | 13% | 8 | 27% | 3 | 10% | Σ | 30 |
| E12.5 | 12 | 16% | 18 | 25% | 9 | 12% | 8 | 11% | 26 | 36% | 0 | 0% | Σ | 73 |
| E14.5 | 15 | 18% | 26 | 31% | 4 | 5% | 13 | 15% | 27 | 32% | 0 | 0% | Σ | 85 |
| E15.5 | 26 | 16% | 48 | 29% | 8 | 5% | 28 | 17% | 56 | 34% | 0 | 0% | Σ | 166 |
| E16.5 | 21 | 17% | 39 | 32% | 9 | 7% | 20 | 16% | 33 | 27% | 0 | 0% | Σ | 122 |
| E18.5 | 21 | 18% | 37 | 32% | 7 | 6% | 17 | 15% | 33 | 29% | 0 | 0% | Σ | 115 |
Analysis of genotypes of live embryos generated from intercrosses of animals that carry wild-type and mutant erbB2 and Nkx2.5erbB2 alleles. The expected Mendelian ratio (expected ratio) for the different genotypes is indicated, and the numbers and percentages of observed live embryos at different developmental stages are shown. Genotypes were identified by PCR analysis on DNA isolated from the yolk sac. In addition to live embryos, embryos without heartbeat or with signs of resorption were observed.