Figure 4.

Complex formation by p6 in the presence of p4 but in the absence of RNA polymerase. DNase I footprinting was performed using the 366-bp DNA fragment. Protein p6 was present at the indicated concentrations and p4 was present at 60 nm. The locations of the p4- and RNAP-binding sites are indicated by lines. Arrows at right indicate DNase I hypersensitivities created on binding of p4 to PA3 numbered relative to the transcription start sites at PA2c/PA3 (the −58 hypersensitivity appears when p4 binds PA3 in the absence of RNAP) and upstream binding of p6 (positions −107 to +41, numbered relative to the transcription start site at PA2c).