Table 1.
Binding of protein p4 and RNA polymerase to PA3 and PA2c in the absence or presence of protein p6
Protein p4 (nm)
|
|||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
330
|
160
|
80
|
40
|
20
|
330
|
160
|
80
|
40
|
20
|
||
|
− protein p6
|
|
+ protein p6
|
||||||||
RNAPA3 | 100 | 89 | 25 | 0 | 0 | 100 | 100 | 96 | 91 | 66 | |
p4A3 | 100 | 85 | 17 | 0 | 0 | 100 | 100 | 100 | 89 | 43 | |
RNAPA2c | 100 | 100 | 100 | 100 | 100 | 0 | 0 | 5 | 10 | 35 | |
p4A2c | 100 | 90 | 14 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Data were obtained by densitometric scanning of the specific bands at positions marked in boxes in Fig. 3. The numbers represent relative percentages obtained after correcting for sample loading variations and background. A value of 100 was arbitrarily assigned to the maximum binding (maximum protection for the +3 band for RNAPA3; maximum hypersensitivities for the bands at −75 for p4A3, −56/−57 for p4A2c, and −37 for RNAPA2c).0 corresponds to no binding, i.e., values equal to background.