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. Author manuscript; available in PMC: 2012 Sep 2.
Published in final edited form as: J Proteome Res. 2011 Jul 28;10(9):4120–4133. doi: 10.1021/pr200310s

Figure 1.

Figure 1

Biochemistry and multivariate data analysis. (A) Liver triglyceride levels in the control (filled triangles) and alcohol-treated (filled diamonds) wild-type mice on the B6 background after one month. The triglyceride levels in their Ppara-null counterparts are represented by empty triangles and diamonds, respectively. (B) Scores scatter plot from unsupervised Principal components analysis (PCA) of the urinary metabolomic signature showing segregation of metabolic fingerprints of control (solid diamonds) and alcohol-treated (empty diamonds) Ppara-null mice on the B6 background at two months. (C) Scores scatter plot from PCA of the urinary metabolomic signature showing distinct metabolic phenotypes (metabotype) associated with chronic alcohol treatment of wild-type and Ppara-null mice on the B6 background at 6 months. The solid triangles, empty triangles, solid diamonds, and empty diamonds represent the control wild-type, control Ppara-null, alcohol-treated wild-type and alcohol-treated Ppara-null mice, respectively. (D) PCA scores scatter plot showing distinct metabotype associated with the mice on the B6 (boxes) and 129S (squares) background throughout the duration of the study irrespective of alcohol exposure status. (E) PCA scores scatter plot illustrating the underlying genetic background-related differences in metabolic traits of the alcohol-treated Ppara-null mice on the B6 or 129S background at 6months. (F) Loadings S-plots from the supervised orthogonal projection to latent structures (OPLS) analysis of the metabolic signatures (at six months) for the selection of candidate markers of chronic alcohol exposure in Ppara-null mice on the B6 background. Each triangle represents an ion characterized by unique mass and retention time. The p(corr)[1] values represent the interclass difference and w(1) values indicate the relative abundance of the ions. Ions shown in boxes represent the potential markers that showed consistent contribution to the interclass difference during alcohol exposure (see Table S2). Those in the upper right and lower left quadrants respectively represent ions with elevated and depleted abundance in the urine of the alcohol-treated B6 Ppara-null mice. Representative results from the positive electrospray ionization (ESI+) mode metabolomic analysis are shown here. Negative electrospray ionization (ESI-) mode also showed similar patterns (data not shown).