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. 1998 Aug 15;12(16):2587–2597. doi: 10.1101/gad.12.16.2587

Figure 1.

Figure 1

Genetic interactions between MET30, CDC34, and SCF components. (A) Herterozygous disruption of MET30 enhances the temperature sensitivity of diploid cdc34-3 mutants. Cells were grown at 23°C, sequentially replica plated to four new plates, and incubated at the temperatures indicated. (B) High-copy suppression. met30-6, cdc53-1, and cdc4-3 mutants were transformed with 2μ-based plasmids harboring no insert (vector), MET30 (2μ-MET30), or CDC34 (2μ-CDC34). Transformants were grown at 23°C and replicas were incubated at 34°C (met30-6 and cdc53-1) or 30°C (cdc4-3). To analyze genetic interactions between MET30 and SKP1, met30-6 mutants expressing SKP1 under control of the GAL1 promoter were grown on glucose plates at 23°C, replica plated to galactose plates to induce expression of SKP1 and incubated at the restrictive temperature of 34°C.