Table 1.

Effects of akt-1(mg144) on dauer formation

Genotype of parent	Phenotype of progeny at 25°C (%)
	L4 larvae and adult	dauer	partial dauer	dead eggs	other	Na
Wild type	100	0	0	0	N.D.	403
akt-1(mg144)	98.4	0	0	1.6	N.D.	314
sqt-1(sc13) age-1(mg44)b	0	82.2	0	3.2	14.6c	185
sqt-1(sc13) age-1(mg44); akt-1(mg144)d,e	88.2	0	0	0.2	11.6f	440
daf-16(m27); sqt-1(sc13) age-1(mg44)d	99.6	0	0	0.4	N.D.	269
unc-4(e120) age-1(m333)b	0	96.5	0	2.3	1.2	171
unc-4(e120) age-1(m333); akt-1(mg144)d,g	96.8	0	0	0	3.2	279
daf-2(e1370)	0	96.1	0	2.8	1.0	387
daf-2(e1370); akt-1(mg144)h	0	0	92.4	0	7.6	436
daf-16(m27); daf-2(e1370)	99.8	0	0	0	0.2	499

(N.D.) Not determined. 

See Materials and methods for description of categories. 

^a(N) Total number of animals scored. Numbers represent the summary of at least two trials of each genotype in at least two experiments performed on different days. 

^bThese animals are the homozygous age-1 progeny of age-1/balancer hermaphrodites. 

^cNine of nine animals scored became dauers by 97 hr post-egg lay. 

^dThese animals are the homozygous age-1 progeny of homozygous age-1 hermaphrodites. 

^eRescoring of the entire assay at 96 hr post-egg lay: 90.5% of animals were gravid adults, 2.7% were sterile adults, 0.7% were dauers, and 6.1% were other (N = 147); sqt-1(sc13) age-1(mg44) animals remained arrested at the dauer stage. 

^fBy 96 hr post-egg lay, 5/28 animals scored became gravid adults, 11/28 became dauers or partial dauers, 10/28 became sterile adults, and 2/28 remained as non-dauer-arrested larvae of various stages. 

^gRescoring of the entire assay at 72 hr post-egg lay: 81.6% of animals were gravid adults, 17.8% were sterile adults, and 0.6% were other (N = 151); unc-4(e120) age-1(m333) animals remained arrested at the dauer stage. 

^hRescoring of the entire assay at 96 hr post-egg lay: 11.5% of animals were gravid adults, 66.3% were sterile adults, 6.9% were dauers, 13.9% were partial dauers, and 1.4% were other (N = 418); daf-2(e1370) animals remained arrested at the dauer stage. In comparison, daf-16(m27); daf-2(e1370) animals formed 96.7% gravid adults, 2.9% sterile adults, and 0.4% other by 72 hr post-egg lay (N = 486).