Table 3.

Effects of akt-1 and akt-2 transgenes on dauer formation

Dauer constitutive mutation	Transgene	Phenotype at 25°C (%)
		L4 larvae and adult	dauer	othera	Nb
Wild typec	none	100	0	0	403
Wild type	akt-1(+)	97.6	0	2.4	454
Wild type	akt-1(mg144)	93.2	0	6.8d	414
Wild type	akt-1(KD)e	98.6	0	1.4	778
Wild type	akt-2(+)	99.3	0	0.7	570
age-1(mg44)c,f	none	0	82.2	17.8	185
age-1(mg44)f	akt-1(+)	66.7	32.0	1.3	228
age-1(mg44)f	akt-1(mg144)	88.3	7.6	4.1	341
age-1(mg44)f	akt-1(KD)e	0	100	0	58
age-1(mg44)f	akt-2(+)	0	100g	0	70
daf-2(e1370)c	none	0	96.1	3.9	387
daf-2(e1370)	akt-1(+)	35.3	63.1h	1.6	510
daf-2(e1370)	akt-1(mg144)	30.3	66.9	2.8	601
daf-2(e1370)	akt-1(KD)e	0	92.5	7.5	375
daf-2(e1370)	akt-2(+)	0	94.8	5.2	289

See Materials and methods for description of categories. 

^aThis category includes dead eggs for the data imported from Table 1. 

^b(N) Total number of transgenic animals scored. Numbers represent the summary of at least two separate trials from two independent transgenic lines of each genotype. 

^cThese data are from Table 1 and were added here for ease of comparison. 

^d72 hrs post-egg lay: Of 17 of these animals, 9 were L4 larvae or adults and 8 remained as non-dauer-arrested larvae of various stages. 

^eakt-1(KD) is a kinase-defective mutation that changes a conserved lysine in the ATP binding site to a methionine and has been shown to cause mammalian Akt/PKB to be a nonfunctional kinase (Franke et al. 1995). 

^fThe full genotype of this strain is sqt-1(sc13) age-1(mg44). 

^g2.9% of these worms were partial dauers (see Materials and Methods for description). 

^h7.4% of these worms were partial dauers (see Materials and Methods for description).