Figure 5.
Role of cytoplasmic readenylation during meiotic maturation. Primary oocytes were injected with the following transcripts (150 ng/μl): Ch-2 (AAUAAA) without [(A)0, lanes 1–4] or with [(A)40, lanes 5–8] a 40-nucleotide poly(A) tail, and a mutant form of Ch-2 lacking a functional polyadenylation signal, Ch-2 (AAGAAA) without [(A)0, lanes 9–12] or with [(A)40, lanes 13–16] a 40-nucleotide poly(A) tail. Injected oocytes were cultured in the presence (GV) or absence of dB-cAMP (BD) and lysed in groups of five after ∼10 hr. Noninjected control oocytes (−) (lanes 17–20) were cultured and lysed in parallel. Synthesis of tPA and uPA were assessed by zymography.