Figure 1.

Twisted gastrulation is a conserved glycoprotein with predicted conserved and non-conserved glycosylation sites. ClustalW aligned proteins from various tetrapods are shown to highlight the high degree of conservation of the Twisted gastrulation protein and to show the locations of conserved and non-conserved glycosylation sites. A protein phylogenetic tree is shown at left, which also correlates with species phylogeny. The alignment of the protein sequences to the mouse is shown at the top. The forth exon deleted in a mouse knockout with craniofacial defects (Petryk et al., 2004) is indicated by a dashed line. The N-glycosylation sequons are indicated in context below with brackets at the bottom indicating the N-X-S sequons, while arrows in yellow or blue at the top indicate the asparagines to which sugars can be linked.