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. 2011 Sep 12;2:59. doi: 10.3389/fphys.2011.00059

Figure 2.

Figure 2

Twisted gastrulation in rodents is N-glycosylated at two residues, both in exon 4. (Top) Locations of mutations that remove or add glycosylation at conserved sites are shown with reference to the sequences in rodents and other tetrapods. (A) Wild type mTWSG1 is glycosylated and runs on SDS-PAGE as a doublet of about 27 kD and 30 kD. (B) Exon 4-deleted mTWSG1 is not glycosylated. (C) The N80Q mutation removes one glycosylation site but is still glycosylated. (D) The N146Q mutation removes one glycosylation site but the protein is still glycosylated. (E) When the N80Q and N146Q mutations are combined the resulting QQ-TWSG1 is not glycosylated. (F) Mutation of a proline in mTWSG1 to the consensus serine restores a third glycosylation site.