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Immunoprecipitation indicates that glycosylation and exon 4 are necessary for BMP binding. Immunoprecipitation (IP) was performed with anti-FLAG and immunoblots (IB) were probed with either anti BMP2 (top) or anti-FLAG (bottom); BMP2 immunoprecipitates with wild type but not glycosylation defective TWSG1QQ or exon 4-deleted TWSG1Δex4.
