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. 1998 Aug 15;12(16):2463–2468. doi: 10.1101/gad.12.16.2463

Figure 4.

Figure 4

RNase P is located primarily in the nucleolus in S. cerevisiae. All panels are stained for nuclear DNA with DAPI (blue). Probes for RNase P RNA or an artificial insert in RNase P RNA (Fig. 3) are shown in green in the left panels. The center panels show probes of the same cells for U14 snoRNA (red). The right panels show the left and center panels merged, with overlap between the green and red signals in yellow. (No merged panel is given for the center set, as there is no RNA signal in the left panel.) (a–c) The mature domain of wild-type RNase P RNA is probed (RPR1 RNA, a,c). (d–e) The probe to an artificial insert in RPR1 RNA is applied to wild-type cells, in which, as expected, it gives no signal above background. (f–h) The artificial insert probe is used (f,h) with cells having the modified RPR1 allele containing inserted sequences complementary to the probe.