Table 1.
Set-up experiments and results on average infection rates. For each experiment (Exp.), the Daphnia clones and parasite strains used and the genes investigated are indicated. Some details of the protocols are also given: parasite doses are given in number of spores per Daphnia, time is given in hours (h) or days (d) and infection rates (0 = no infection, 1 = all Daphnia infected) are given for each clone (conserved order).
| Exp. | Figure | Daphnia clone | Parasite | Dose | Exposure time | Time points | Genes investigated | Infection rate |
|---|---|---|---|---|---|---|---|---|
| A | Fig. 1 | GG4/GG7 | Sp1 | 100,000 | 2 h | 0 h, 1 h, 2 h, 6 h, 12 h, 24 h, 48 h | actin, GAPDH, arginase, proPO, NOS1, NOS2 | 0.73/0 |
| B | Fig. 1 | GG4/GG7/GG13 | Sp1 | 100,000 | 2 h | 0 h, 1 h, 2 h, 6 h, 12 h, 24 h, 48 h | actin, GAPDH, arginase, proPO, NOS1, NOS2 | 1/0/0 |
| C | Fig. 2 | GG4/GG7/GG13 | Sp8 | 150,000 | 2 h | 0 h, 1 h, 2 h, 4 h, 6 h, 12 h, 24 h, 48 h | actin, GAPDH, arginase, proPO, NOS1, NOS2 | 0/0/0.27 |
| D | Fig. 3 | 11.3/12.2 | Cl25.5_P2.1R3 | 100,000 | 2 h | 0 h, 1 h, 2 h, 6 h, 12 h, 24 h, 48 h | actin, GAPDH, proPO, NOS1, α2M | 0.91/0.61 |
| E | Fig. 4 | GG4/GG7 | Sp1 | 20,000 | 48 h | 12 h, 24 h, 2d, 6d, 8d, 16d | actin, GAPDH, arginase, proPO, NOS1, NOS2 | 1/0 |
| F | Fig. 5 | GG4 | Sp1 | 100,000 | 2 h | 2 h | actin, GAPDH, proPO, NOS1, α2M | 1 |