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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: J Immunol. 2011 Apr 29;186(11):6173–6181. doi: 10.4049/jimmunol.1100264

FIGURE 3.

FIGURE 3

Serum IL-21 levels and IL-21R expression in B lymphocyte subsets in HC and HIV-infected patients pre- and post-H1N1 vaccination: serum levels of IL-21 were measured by ELISA (A). Fresh PBMC (1 × 106 cells) were stained with surface markers for B cell subset characterization (CD20, CD21, CD10, CD27) and for IL-21R. Total B cells and various B cell subsets were analyzed at baseline (T0), 7 d (T1), and 28 d (T2) postvaccination for IL-21R by flow cytometry. Data are shown for T0 and T2 as no changes were noted at T1 compared with T0. B, Representative histograms showing frequencies and MFI (in parentheses) of IL-21R expression in total B cells of eight HC, eight HIV-infected H1N1 Ab responders, and eight HIV-infected H1N1 Ab nonresponders at T0 (gray) and T2 (black) compared with isotype controls (filled). C, Box plots are shown to illustrate frequency of IL21R expression in total B cells. D, Correlation of H1N1 Ab titer with percentage of IL21R+ B cells at T2 in HIV-infected patients. E, Frequency of IL21R expression B cell subsets at baseline. Box plots include median with 25th and 75th percentile borders, and error bars represent 10th and 90th percentiles. Stars indicate the level of significance. ★p < 0.05, ★★p < 0.01, ★★★p < 0.001.