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. Author manuscript; available in PMC: 2011 Sep 12.
Published in final edited form as: Nature. 2011 Jan 26;470(7332):95–100. doi: 10.1038/nature09669

Figure 2. The adult zebrafish kidney contains transplantable progenitors that form functional nephrons.

Figure 2

a, Overview of the transplantation assay. b, A primary transplanted fish at 18 d.p.t. with cdh17:EGFP+ donor-derived nephrons (arrow; inset, higher magnification view; scale bar, 0.5 mm). c, Average number of donor-derived nephrons over time (error bar, one standard deviation; n, total fish per time point). d, Head kidney of a recipient at 34 d.p.t. showing expansion of renal tissue caused by cdh17:mCherry+ donor-derived nephrons (arrow; scale bar, 0.5 mm). e, A cdh17:mCherry+ donor-derived nephron showing functional uptake of 40 kDa FITC-conjugated dextran (scale bar, 30 μm). f, Connection of donor-derived nephrons (cdh17:mCherry+) with the cdh17:EGFP+ recipient’s renal system (scale bar, 10 μm). g, A mosaic nephron arising from the co-injection of a mixture of cdh17:EGFP- and cdh17:mCherry-labelled nephron progenitors. h, Overview of the serial transplantation assay. i–k, Donor-derived nephrons (cdh17:EGFP+, arrows) in primary-, secondary- and tertiary-engrafted recipients (scale bar, 0.5 mm).