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. Author manuscript; available in PMC: 2011 Sep 12.
Published in final edited form as: Nature. 2011 Jan 26;470(7332):95–100. doi: 10.1038/nature09669

Figure 4. lhx1a:EGFP+ cells form nephrons during adult kidney development and after transplantation.

Figure 4

a, Lateral view of a Tg(lhx1a:EGFP;cdh17:mCherry) larva showing the first lhx1a:EGFP+ cell to appear on top of the cdh17:mCherry+ embryonic kidney tubules (arrow and inset). b, Lateral view of a Tg(lhx1a:EGFP) larva showing the extent of lhx1a:EGFP+ cell migration (arrows) and their aggregation (arrowheads). c, Laser-ablation of an lhx1a:EGFP+ aggregate (arrow) inhibits nephron formation without affecting nephrogenesis of an adjacent aggregate (arrowhead). d, Time course of a Tg(lhx1a:EGFP;cdh17:mCherry) larva demonstrating that lhx1a:EGFP+ cells coalesce into an aggregate and differentiate into a nephron. e, Time course of a Tg(wt1b:EGFP;pax8:DsRed) larva showing development of a wt1b:EGFP+ aggregate into a nephron. f, Bright field and fluorescent merge of an aggregate from a Tg(lhx1a:EGFP;cdh17:mCherry) donor. g, Donor-derived cdh17:mCherry+ nephrons (one indicated by arrow) and multiple single lhx1a:EGFP+ cells (arrowhead) resulting from the transplantation of the aggregate shown in f (scale bar, 30 μm). PT, pronephric tubule; sb, swim bladder; larvae shown with anterior to the left.