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. 2000 Dec 1;14(23):2944–2949. doi: 10.1101/gad.848200

Figure 5.

Figure 5

Effect of Ran on protein/RNA complex formation and cap methylation. (A) Samples containing 1 μM MT (lane 1) plus 1 μM Impα (lane 2), plus 3 μM Impβ (lane 3), plus 5μM RanGTP (lane 4) or RanGDP (lane 5) were incubated with GpppG-RNA and assayed by gel shift as in Fig. 3. (B) Samples containing 3 nM MT (column 1); 3nM MT, 30 nM Impα (column 2); 3 nM MT, 30 nM Impα, 120 nM Impβ (column 3); 3nM MT, 30 nM Impα, 120 nM Impβ, 200 nM RanGTP (column 4) or RanGDP (column 5), were assayed for cap methylation as in Fig. 4.