Characterization of ischemic lesion with multiparametric perfusion, diffusion, and pH-weighted APT MRI of a representative acute stroke animal with MCAO. The animal showed a large perfusion and diffusion lesion mismatch, whereas the pH-weighted APT lesion agreed reasonably well with the diffusion deficit. ADC, apparent diffusion coefficient; APT, amide proton transfer; CBF, cerebral blood flow; CESTRasym, chemical exchange saturation transfer ratio asymmetry; MCAO, middle cerebral artery occlusion; MRI, magnetic resonance imaging.