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. 2000 Dec 1;14(23):2976–2988. doi: 10.1101/gad.188700

Figure 4.

Figure 4

Effect of the location of dif on the level of HJs. (A) HJs at dif (indicated by arrowheads) were compared in three strains carrying a single site in DAZ (LN3080), at 150 kb to the left side of DAZ (zda192, LN3577) or at 1 Mb to the right side of DAZ (lacZ, LN3091). The positions of the two sister dif sites in dividing cells are represented below the blots. Numbers indicate the relative intensity of the HJ spot to the intensity of the smaller linear fragment (mean of two independent experiments). (B) Increased and location-independent levels of HJ in cells carrying two tandem dif sites. HJ levels were compared in FtsKc− and XerC− cells carrying a dif-Km-dif cassette in DAZ or in lacZ (FC313, FC354, FC235, FC284). EcoRV (E) and HindIII (H) were used to restrict DNA. The restriction digest map of the dif-Km-dif cassette and the molecules that can be attributed to the a, b, c, and d spots are represented below the blots. Note that the intensity of spot d must be compared with the sum of the intensities of spot a and b.