Table 1.
Strains and plasmids
| Strain
|
Relevant genotype
|
Source/reference
|
|---|---|---|
| AB1157 | Rec+ laboratory strain | Summers and Sherratt 1988 |
| DS941 | AB1157 recF | Summers and Sherratt 1988 |
| DS9041 | DS941 ftsK∷CmR | Recchia et al. 1999 |
| DS8029 | AB1157 xerC∷KmR | G. Blakely |
| GR18 | AB1157 xer∷KmR | G. Recchia |
| MA3 | AB1157 ftsK∷CmR | Recchia et al. 1999 |
| GR19 | AB1157 recA∷CmR | Recchia et al. 1999 |
| N3137 | AB1157 uvrA∷Tn10 | McGlynn and Lloyd 2000 |
| JJC40 | AB1157 hsdR | Seigneur et al. 1998 |
| JJC662 | JJC40 ruvA60∷Tn10 | Michel et al. 2000 |
| JJC494 | JJC662 Δrep∷KmR | Seigneur et al. 1998 |
| JJC733 | JJC662 xerC∷KmR | Michel et al. 2000 |
| LN3080 | LN2666 (thy−) dif in DAZ | Cornet et al. 1996 |
| LN3091 | LN2666 dif in LacZ | Perals et al. 2000 |
| LN3577 | LN2666 dif in zda192 | Perals et al. 2000 |
| FC313 | AB1157 ftsK dif-Km-dif in DAZ | F. Cornet |
| FC354 | AB1157 ftsK dif-Km-dif in lacZ | F. Cornet |
| FC235 | LN2666 xerC dif-Km-dif in DAZ | F. Cornet |
| FC284 | LN2666 xerC dif-Km-dif in lacZ | F. Cornet |
| GR59 | MA3 recA56 srl∷Tn10 | Recchia et al. 1999 |
| FC217 | FC235 recA | F. Cornet |
| NL250 | DS941 difΔ6 dif in lacZ | Leslie and Sherratt 1995 |
| MA2 | NL250 ftsK | Recchia et al. 1999 |
| Plasmid | Description | Source/reference |
| pBAD24 | Expression vector | Guzman et al. 1995 |
| pFX83 | Flag fusion vector | This study |
| pFX88 | fl FtsK N-terminally tagged | This study |
| pFX89 | 734 N-terminally tagged | This study |
| pFX90 | 818 N-terminally tagged | This study |
| pFX91 | 855 N-terminally tagged | This study |
| pFX99 | 906 N-terminally tagged | This study |
| pFX101 | 1260 N-terminally tagged | This study |
| pFX102 | ATP− N-terminally tagged | This study |
| pSDC124 | pUC18-based dif reporter | Blakely et al. 1991 |
| pFC238 | pGB2-based dif reporter | Recchia et al. 1999 |
| pFH127 | pGB2ts carrying one dif site | Hayes and Sherratt 1997 |
| pFC225 | p15a-based XerC expression vector | F. Cornet |
DAZ, dif activity zone; fl, full length.