Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1999 Oct 15;13(20):2691–2703. doi: 10.1101/gad.13.20.2691

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 1999, Cold Spring Harbor Laboratory Press

PMC Copyright notice

Hemopoietic parameters of Bmi1 mutant and eed/Bmi1 double mutant mice analyzed at 12–16 weeks of age. (A) Bone marrow, spleen, and thymic cellularity (left) and bone marrow CFC numbers (right) in control (n = 9), Bmi1^+/− (n = 7), Bmi1^−/− (n = 3), eed^+/− Bmi1^−/− (either eed^3354/+ or eed^1989/+; n = 3), and eed/Bmi1^+/− (either eed^1989/+ or eed^1989/1989; n = 13) mutant mice. For clarity, the results for single C3Hf/101 × FVB/N eed mutant mice were excluded from this table because the data are superimposable to that of the C3Hf/101 eed mutant animals presented in Fig. 2B. (B) Frequency and proliferative potential of primitive myeloid (LTC-IC) and primitive lymphoid (WW-IC) bone marrow cells in Bmi1 vs. eed/Bmi1 double mutant mice. All results shown in A and B represent mean values ± SD. (*) Not determined. (NC) Nucleated cells; (HPP-CFC) high proliferative potential–CFC. ^aAbsolute number of B220⁺ cells analyzed by FACS at day 21 of in vitro culture; reflects the proliferative potential of individual WW-IC. ^b Absolute number of CFC per LTC-IC; reflects the proliferative potential of individual LTC-IC. ^c One eed^1989/+ Bmi1^−/− and two eed^3354/+ Bmi1^−/− mutant mice were included in this calculation because they had very similar phenotypes. ^d Includes both eed^1989/+ Bmi1^+/− and eed^1989/1989Bmi1^+/− double mutant mice because they had very similar phenotypes. ^e The values corresponding to the Bmi1^+/− and eed/Bmi1^+/− groups of mice are not overlapping, but because of an extreme value obtained in one eed/Bmi1^+/− mouse (1980 B cells/WW-IC), they are not statistically different (P < 0.07, one-tailed Student's t-test with unequal variance).

Hemopoietic parameters of Bmi1 mutant and eed/Bmi1 double mutant mice analyzed at 12–16 weeks of age. (A) Bone marrow, spleen, and thymic cellularity (left) and bone marrow CFC numbers (right) in control (n = 9), Bmi1^+/− (n = 7), Bmi1^−/− (n = 3), eed^+/− Bmi1^−/− (either eed^3354/+ or eed^1989/+; n = 3), and eed/Bmi1^+/− (either eed^1989/+ or eed^1989/1989; n = 13) mutant mice. For clarity, the results for single C3Hf/101 × FVB/N eed mutant mice were excluded from this table because the data are superimposable to that of the C3Hf/101 eed mutant animals presented in Fig. 2B. (B) Frequency and proliferative potential of primitive myeloid (LTC-IC) and primitive lymphoid (WW-IC) bone marrow cells in Bmi1 vs. eed/Bmi1 double mutant mice. All results shown in A and B represent mean values ± SD. (*) Not determined. (NC) Nucleated cells; (HPP-CFC) high proliferative potential–CFC. ^aAbsolute number of B220⁺ cells analyzed by FACS at day 21 of in vitro culture; reflects the proliferative potential of individual WW-IC. ^b Absolute number of CFC per LTC-IC; reflects the proliferative potential of individual LTC-IC. ^c One eed^1989/+ Bmi1^−/− and two eed^3354/+ Bmi1^−/− mutant mice were included in this calculation because they had very similar phenotypes. ^d Includes both eed^1989/+ Bmi1^+/− and eed^1989/1989Bmi1^+/− double mutant mice because they had very similar phenotypes. ^e The values corresponding to the Bmi1^+/− and eed/Bmi1^+/− groups of mice are not overlapping, but because of an extreme value obtained in one eed/Bmi1^+/− mouse (1980 B cells/WW-IC), they are not statistically different (P < 0.07, one-tailed Student's t-test with unequal variance).