Fig. 2.

Flow cytometry analysis of primary natural killer (NK) cells cultured in the presence of hepatitis C virus (HCV). NK cells from normal donor 1 were cultured for 5 days in Iscove's modified Dulbecco's medium/interleukin-2 (IMDM/IL-2) medium alone, in IMDM supplemented with HCV pseudoparticles (HCVpp)-containing supernatant, or in IMDM supplemented with HCV1b-containing human sera. Cells were stained with AlexaFluor 647-conjugated anti-CD16 and either anti-NKp44-phycoerythrin (PE), anti-CD94-PE or anti-CD81-PE monoclonal antibodies, respectively.