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Binding rate constants and affinities of scFv m708.2,5 as measured by FACS and Biacore.
| Antibody | Antigen | FACSa | Biacoreb | ||
|---|---|---|---|---|---|
| KD (M) | kon (M−1s−1) | koff (s−1) | KD(M) | ||
| m708.2 scFv | IGF1 | 3.9×10−9 | 1.1×106 | 2.4×10−3 | 2.2×10−9 |
| IGF2 | 1.1×10−9 | 2.3×105 | 4.0×10−4 | 1.8×10−9 | |
| m708.5 scFv | IGF1 | 1.0×10−10 | 1.4×106 | 2.8×10−4 | 2.0×10−10 |
| IGF2 | 4.1×10−11 | 4.1×106 | 2.5×10−6 | 6.1×10−11 | |
Values for yeast-displayed scFvs.
Values for purified soluble scFvs.
