Table 2.
Antibody | Antigen | FACSa | Biacoreb | ||
---|---|---|---|---|---|
KD (M) | kon (M−1s−1) | koff (s−1) | KD(M) | ||
m708.2 scFv | IGF1 | 3.9×10−9 | 1.1×106 | 2.4×10−3 | 2.2×10−9 |
IGF2 | 1.1×10−9 | 2.3×105 | 4.0×10−4 | 1.8×10−9 | |
m708.5 scFv | IGF1 | 1.0×10−10 | 1.4×106 | 2.8×10−4 | 2.0×10−10 |
IGF2 | 4.1×10−11 | 4.1×106 | 2.5×10−6 | 6.1×10−11 |
Values for yeast-displayed scFvs.
Values for purified soluble scFvs.