Skip to main content
. 2000 Dec 1;14(23):2950–2961. doi: 10.1101/gad.183500

Figure 2.

Figure 2

ADDER (amplification of double-stranded cDNA end restriction fragments) differential display of liver cDNAs from mice fed exclusively during the day or during the night. Double-stranded cDNA fragments encompassing mRNA sequences located between the poly A addition sites and the most proximal Mbo1 restriction sites were synthesized using total liver RNA as a template. The RNA was collected at 4-h intervals from mice fed either during the day or during the night. The left panel shows a subpopulation of cDNAs containing the 3′ sequences of coumarin 7 hydroxylase (Cyp2a5) transcripts and ∼60 other 3′-terminal mRNA sequences amplified and displayed on a 5% urea-polyacrylamide gel (see Materials and Methods). X is a yet unidentified circadian fragment. The right panel shows a subpopulation of cDNA amplified using a second set of primers (see Materials and Methods). Y is a yet unidentified circadian fragment. The positions of molecular size markers are indicated in the middle of the figure.