Figure 6.

Restricted feeding resets the phase in various peripheral tissues. Mice kept under a light-dark regimen (lights on 6 a.m., lights off 6 p.m.) were fed for three or six consecutive days exclusively during the light phase (6 a.m. to 6 p.m.) or for six days exclusively during the dark phase (6 p.m. to 6 a.m.). During the fourth or seventh day, respectively, animals were sacrificed at 4-h intervals to prepare whole-cell RNA from liver, kidney, heart, and pancreas. Dbp mRNA levels were determined by ribonuclease protection assays in the organs and at the daytimes indicated on top of the panels. β-actin mRNA was included as a transcript whose accumulation does not oscillate during the day. (A) Temporal Dbp expression in mice fed during 6 d exclusively during the night. (B) Temporal Dbp expression in mice fed during 6 d exclusively during the day. (C) Temporal Dbp expression in mice fed during 3 d exclusively during the day.