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. 2011 Aug 9;105(6):824–832. doi: 10.1038/bjc.2011.283

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Copyright © 2011 Cancer Research UK

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Effect of Ln5-γ2 siRNA on tumour cell invasion examined by the raft culture assay. (A) Histological findings on days 2 and 6 of raft culture. Human A431 SCC cells invaded the fibroblast-embedded collagen gel, while HaCaT normal keratinocytes did not. (B, C) Histological findings of invading tumour cells. The invading tumour cell layers were thicker in non-treated or control siRNA-treated cells than Ln5-γ2 siRNA-treated ones. Ln5-γ2 expression also reduced in the invasion front. (D) Tumour invasion analysed at day 6 of raft culture and expressed as area occupied by invading SCC cells. Treatment with Ln5-γ2 siRNA significantly reduced the area of the invading tumour cells. Data are shown as mean±s.e.m. of six experiments. (A) Hematoxylin and Eosin staining, (B) immunohistochemistry with anti-pancytokeratin (AE1/AE3), (C) immunohistochemistry with anti-human Ln5-γ2 monoclonal antibody.