Figure 4.

HAC1 is a negative regulator of early meiotic gene expression. (A,C) ime2–lacZ expression after induction of meiosis with C-SPO medium. The average and standard error of three independent clones (A) or experiments (C) are shown. (A) WT cells (AMP1618 and AMP1619) were transformed with an empty plasmid (pRS316) or with a plasmid that carries the HAC1ⁱ gene under control of its own promoter (pRS316-HAC1ⁱ). Uracil was omitted from acetate (PSP2) and C–SPO medium. The black and open bars represent two independent experiments. (C) WT (AMP1619), ire1Δ (MSY18-59), and hac1Δ cells (MSY20-19) were grown on YPAc and shifted to C–SPO medium. (B,D) Northern analysis to the experiments described in A and C, respectively. All lanes were taken from the same blot with the same exposure time. Two mRNAs for HOP1 and SPO13 were detected, as reported by Smith et al. (1990). Similar results were obtained in two independent experiments.