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. 2000 Dec 1;14(23):2962–2975. doi: 10.1101/gad.852300

Figure 6.

Figure 6

Pseudohyphal growth persists during meiosis in ire1Δ/ire1Δ and hac1Δ/hac1Δ strains. (A) Time course of ascus formation by homozygous diploid WT (filled squares), ire1Δ (open squares), and hac1Δ (filled circles) strains. (B,D) Meiosis was induced in homozygous diploid WT, ire1Δ, and hac1Δ strains grown to mid-log phase on glucose (D) or acetate (B) by shifting to C–SPO medium. Cells were photographed and counted before (filled bars) and 30 (D) or 20 h (B; open bars) after induction of meiosis. Representative pictures of the majority of the population after induction of meiosis are shown. Abbreviations: YF, yeast form (length to width [l/w] ratio less than 2), PH, pseudohyphal cells (l/w greater than or equal to two). More than 100 cells were counted for each strain. (C) Sporulation efficiency after 5 d. Growth to mid-log phase before induction of meiosis was on glucose (filled bars) or acetate (open bars). The average and standard error of two experiments are shown.