Skip to main content
. 1999 Oct 15;13(20):2658–2669. doi: 10.1101/gad.13.20.2658

Figure 6.

Figure 6

ARF deletion and Mdm2 expression in tumors arising in ARF+/− and ARF−/− Eμ–myc mice. (A) Southern blot analysis of pre-B and B-cell tumors. Genomic DNAs digested with AflII were hybridized with a probe that detects a 7.8-kb fragment containing ARF exon1β and a mutant 6.0-kb fragment in which the exon was disrupted. The positions of fragments derived from the wild-type and mutant alleles are indicated at the left. Tail DNAs from wild-type, ARF+/−, or ARF null mice (lanes 14–16) were run as controls. (B) Tumors from the same mice shown in A (with corresponding lane numbers) were analyzed for Mdm2 expression as in Fig. 4. Immunoblots were probed with Mdm2-specific antibody C-18 to detect p92, p90, and p85 isoforms. (C) Tumors from ARF−/− Eμ–myc animals were analyzed for Mdm2 expression as in B. Extracts of MEFs lacking both p53 and Mdm2 were used as a negative control.