Figure 7.

¹H-¹⁵N HSQC demonstrating that Cx26CL interacts with both taurine and the Cx26CT. Pairwise interactions between CL peptide and taurine or CL peptide were reassessed by NMR. Control spectrum of the [¹⁵N]-labeled Cx26CL (10 µM; black) overlaid with spectra of the Cx26CL in the presence of (A) 30 mM taurine (red) or (B) 4 mM Cx26CT peptide (red). (Right) Close-up view of representative resonance peaks of the Cx26CL that were unaffected (i and ii) and affected (iii–vi) by the presence of these ligands. Resonance peaks iii and iv were affected by taurine, but resonance peaks v and vi were not, whereas resonance peaks iii–vi were affected by the Cx26CT peptide.