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. 2000 Dec 1;14(23):3003–3013. doi: 10.1101/gad.848800

Figure 3.

Figure 3

Distribution of phosphorylated H3 changes during heat shock. (A–C) FITC image of anti-Ser 10 phosphorylated H3 immunostaining. (D–F) 4′,6-diamidino-2-phenylindole (DAPI) counterstaining of chromosomes shown in (A–C). (G–I) Composite merge of FITC and DAPI channels, antibody staining shown in yellow, DAPI shown in blue. (A,D,G) Antibodies specific for Ser 10 phosphorylated H3 stain multiple discrete regions of polytene chromosomes isolated from third instar larvae at 22°C, including several of the indicated ecdysone-induced developmental puffs. (B,E,H) After heat shock, phosphorylated H3 staining is restricted to subdivisions 87AC, 63BC, and 67B, which contain the hsp70 gene cluster, hsp83 gene, and the small hsp gene cluster (hsp22, hsp23, hsp26, and hsp27), respectively. (C,F,I) Image of a section of chromosome 3R showing the change in staining of three of the heat shock loci before and after heat shock. Heat shocked chromosome is on the right.