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. 1999 Oct 15;13(20):2725–2737. doi: 10.1101/gad.13.20.2725

Figure 2.

Figure 2

Figure 2

Figure 2

Cleavage requires D269 and E392 from the R1 trans subunit. (A) Schematic depicting the location of each subunit relative to the cleavage site (arrow) on the bio Mu DNA end. (B, C) SDS-PAGE of cross-linked (and therefore labeled) complexes from the total reaction (Total) and complexes released from the matrix upon cleavage of the bio substrate (Cleaved). The entire gel is shown only for R1 trans and R1 cis Total (wells at top). Percent DDE+ transposase cross-linked to each site is shown below each panel. The molar ratio of DDE+ to DDE− initially included in each reaction is indicated above each lane in B, Total; equivalent lanes in all other panels included the same ratio. Transpososomes were cross-linked before (C) or after (B) the cleavage step.