Table 1.

Cleavage requires D336 from the R1 trans subunit^a

Input ratio of DDE+ to DDE− transposaseb	Percent of total protein cross-linked to the Mu DNA end binding site that was DDE+ (%DDE+)
	R1 trans		R2 trans
	Total	Cleaved	Total	Cleaved
1:1	33 ± 4	91 ± 2	53 ± 2	56 ± 2
1:3	18 ± 2	84 ± 8	29 ± 2	32 ± 2
1:5	13 ± 2	81 ± 10	21 ± 4	24 ± 2
	R1 cis		R2 cis
	Total	Cleaved	Total	Cleaved
1:1	31 ± 4	31 ± 2	47 ± 2	50 ± 1
1:3	18 ± 1	19 ± 1	25 ± 5	30 ± 1
1:5	13 ± 1	14 ± 1	17 ± 6	23

^aNumbers ± 1 s.d. are the average of four to five experiments for trans and are the average of two experiments for cis. Experiments were performed as described in Figs 1 and 2; complexes were cross-linked before or after cleavage. 

^bDDE⁺ MuA = 77-605, DDE⁻ MuA = 77-663,D336N.