Table 2.
Sequential cleavage and strand transfer of one Mu DNA end requires DDE residues only from the R1 trans subunita
| Input ratio of DDE+ to DDE− transposaseb
|
Percent of total protein cross-linked to the Mu DNA end binding site that was DDE+ (%DDE+)
|
|||
|---|---|---|---|---|
| R1 trans
|
R2 trans
|
|||
| Total
|
STC
|
Total
|
STC
|
|
| 1:1 | 56,54 | 100,100 | 60,63 | 69,73 |
| 1:3 | 32,34 | 100,100 | 35,40 | N.A.,55 |
| 1:5 | 23,29 | 100,100 | 28,31 | 42,32 |
| R1 cis | R2 cis | |||
| Total | STC | Total | STC | |
| 1:1 | 57,68 | 74,54 | 60,62 | 69,79 |
| 1:3 | 32,37 | 51,26 | 34,38 | 37,51 |
| 1:5 | 22,24 | 35,18 | 24,25 | 31,36 |
a
Numbers from two experiments are shown. Experiments were as described in Fig. 4.
b
DDE+ = 77-605, DDE− = 77-663,DE/NQ.