Figure 4.

The effect of various inhibitors on iso-osmotic water transport (J_v) in the intestine of rainbow trout. In (A) trout were acclimated to either FW or SW and non-everted sacs of the posterior intestine were used for the measurements of J_v (shown in μL cm⁻² h⁻¹): ouabain (Ouab) was added to the serosal side; bumetanide (Bumet), and KCN were added to the mucosal side. Following the control measurement, intestinal sacs were incubated for 30 min with the test agent before the effect was evaluated. All inhibitors were applied at 10⁻⁴ M concentrations. In (B) whole (i.e., middle + posterior) intestines from SW trout were used to measure J_v (shown in % of control): phloridzin (1 mmol L⁻¹) or TEA (10 mmol L⁻¹) were added to the mucosal side. Asterisk indicates difference from control value (ringer; p < 0.05). All values represent mean ± SEM, n = 6–8.