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. 2011 Sep 7;2:56. doi: 10.3389/fphys.2011.00056

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Copyright © 2011 Madsen, Olesen, Bedal, Engelund, Velasco-Santamaría and Tipsmark.

This is an open-access article subject to a non-exclusive license between the authors and Frontiers Media SA, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and other Frontiers conditions are complied with.

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Fluorescent microscope images (40× objective) showing localization of Aqp1aa (A), Aqp1ab (B), and Aqp8ab (C) in pyloric caeca from SW-acclimated Atlantic salmon. Sections were incubated with antibodies against the aquaporin (red), Na⁺, K⁺-ATPase (green), and nuclei were counterstained with DAPI (blue). Lumen, goblet cells, nuclei, brush border (bb), and sub-apical vesicles (sav) are indicated. Note the distinct immunoreaction to all three aquaporins in the brush border and for Aqp1aa and −1ab also in the sub-apical cytoplasmic domain. Size bar = 20 μm.