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. 2011 Sep 7;2:56. doi: 10.3389/fphys.2011.00056

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Copyright © 2011 Madsen, Olesen, Bedal, Engelund, Velasco-Santamaría and Tipsmark.

This is an open-access article subject to a non-exclusive license between the authors and Frontiers Media SA, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and other Frontiers conditions are complied with.

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Confocal images (63× objective) showing localization of Aqp1ab in middle (A) and posterior segments (B,C) of intestine from SW-acclimated Atlantic salmon. (B,C) are from the proximal and distal region of the posterior segment, respectively, with reference to the ileo-rectal valve. Sections were incubated with a cocktail of antibodies against Aqp1ab (red), Na⁺, K⁺-ATPase (green), and nuclei were counterstained with DAPI [blue, only in (C)]. Lumen, goblet cells, nuclei, brush border (bb), and sub-apical vesicles (sav) are indicated. Size bar = 20 μm.