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Confocal images (63× objective) showing localization of Aqp8ab in middle (A,B) and posterior (C,D) segments of intestine from SW-acclimated Atlantic salmon. Sections were incubated with a cocktail of antibodies against Aqp8ab (red), Na+, K+-ATPase (green), and nuclei were counterstained with DAPI [blue, in (A–C)]. Lumen, goblet cells, nuclei, brush border, (bb) and sub-apical vesicles (sav) are indicated. Size bar = 20 μm.
