Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Sep 5;194(5):789–805. doi: 10.1083/jcb.201103168

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 Rohn et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 5. — RBX1 depletion reduces active, GTP-bound Rac1. PAK-PBD bead pull-down of HeLa cell lysates depleted for control, RBX1, or Rac1 by siRNA, compared with the corresponding cellular lysate, probed with α-Rac1. (A) The mean of three independent experiments, normalized to control levels, is shown. Error bars show standard deviation; B shows a representative blot. The reaction control samples, preloaded with GTPγS and GDP (rightmost two lanes), were run in parallel but on a separate gel and membrane, as indicated.