Table I.
Final list of genes with actin phenotypes conserved between Drosophila and mammalian cells
| FBgn | Gene | Phenotype group | Known actin regulator | Validated | Function | Mammalian orthologue(s) | HeLa actinome hit(s) | Mouse nuclear hit(s) |
| FBgn0015610 | Caf1 | High actin | No | Yes | Chromatin remodeling | RBBP4 | No | N/A |
| FBgn0034577 | cpa | High actin | Yes | Yes | F-actin capping | CAPZA1, CAPZA2, CAPZA3 | CAPZA1, CAPZA3 | N/A |
| FBgn0011570 | cpb | High actin | Yes | Yes | F-actin capping | CAPZB | CAPZB | N/A |
| FBgn0000042 | Act5C | Low actin | Yes | Yes | Actin filament formation | ACTG1 | ACTG1 | N/A |
| FBgn0000308 | chic | Low actin | Yes | Yes | G-actin binding | PFN1, PFN2, PFN3, PFN4 | PFN1, PFN4 (PFN3 not screened) | N/A |
| FBgn0011785 | BRWD3 | Other actin | No | Yes | WD40 domain protein | BRWD1,BRWD3, PHIP | No | N/A |
| FBgn0028388 | Capt | Other actin | Yes | Yes | G-actin binding | CAP1, CAP2 | No | N/A |
| FBgn0035586 | CG10671 | Other actin | No | No | Endoplasmic reticulum | FITM1, FITM2 | No | N/A |
| FBgn0039205 | CG13623 | Other actin | No | Yes | Mitochondrial | ISCA2 (HBLD1) | ISCA2 | N/A |
| FBgn0001491 | L(1)10Bb | Other actin | No | Yes | GPCR signaling | BUD31 (G10) | BUD31 | N/A |
| FBgn0044826 | Pak3 | Other actin | Yes | Yes | Rac GTPase signaling | PAK3 | No | N/A |
| FBgn0021967 | Pdsw | Other actin | No | No | Mitochondial electron transport chain | NDUFB10 | No | N/A |
| FBgn0011726 | tsr | Other actin | Yes | Yes | F-actin severing | CFL1, CFL2, DSTN | CFL1, DSTN | N/A |
| FBgn0052138 | CG32138 | Spiky | Moderately | Yes | Uncharacterized formin family member | FMNL1, FMNL2, FMNL3 | FMNL1 | N/A |
| FBgn0002183 | dre4 | Spiky | No | Yes | DNA repair | SUPT16H | SUPT16H | N/A |
| FBgn0015509 | lin19 | Spiky | No | Yes | Ubiquitin-dependent protein degradation | CUL1 | Not screened | N/A |
| FBgn0040291 | Roc1b | Spiky | No | Yes | Ubiquitin-dependent protein degradation | RBX1 | RBX1 | N/A |
| FBgn0003415 | skd | Spiky | No | Yes | Mediator complex | MED13, MED13L | No | N/A |
| FBgn0005411 | U2af50 | Spiky | No | Yes | RNA splicing | U2AF2 | U2AF2 | N/A |
| FBgn0020510 | Abi | Spiky (SCAR-like) | Yes | Yes | SCAR complex | ABI1, ABI2 | No | N/A |
| FBgn0031781 | Arc-p20a | Spiky (SCAR-like) | Yes | Yes | ARP2/3 complex | ARPC4 | ARPC4 | N/A |
| FBgn0011742 | Arp14D | Spiky (SCAR-like) | Yes | Yes | ARP2/3 complex | ACTR2 | No | N/A |
| FBgn0011744 | Arp66B | Spiky (SCAR-like) | Yes | Yes | ARP2/3 complex | ACTR3 | No | N/A |
| FBgn0039754 | CG9747 | Spiky (SCAR-like) | No | Yes | Fatty acid desaturase | SCD, SCD5, TRMT2A, TRMT2B | SCD (only one screened) | N/A |
| FBgn0011771 | Hem | Spiky (SCAR-like) | Yes | Yes | SCAR complex | NCKAP1 | NCKAP1 | N/A |
| FBgn0031437 | p16-arc | Spiky (SCAR-like) | Yes | Yes | ARP2/3 complex | ARPC5 | No | N/A |
| FBgn0010333 | Rac1 | Spiky (SCAR-like) | Yes | Yes | Rho family GTPase | RAC1 | RAC1 | N/A |
| FBgn0014011 | Rac2 | Spiky (SCAR-like) | Yes | Yes | Rho family GTPase | RAC2 | No | N/A |
| FBgn0041781 | SCAR | Spiky (SCAR-like) | Yes | Yes | SCAR complex | WASF1, WASF2, WASF3 | WASF3 | N/A |
| FBgn0016984 | Sktl | Spiky (SCAR-like) | Yes | Yes | Lipid kinase | PIPK51A, PIPK51B, PIPK51C | PIP5K1C | N/A |
| FBgn0023423 | slmb | Spiky (SCAR-like) | No | Yes | Ubiquitin-dependent protein degradation | BTRC, FBXW11 | Not screened | N/A |
| FBgn0038320 | Sra-1 | Spiky (SCAR-like) | Yes | Yes | SCAR complex | CYFIP1, CYFIP2 | CYFIP1 | N/A |
| FBgn0032859 | Arc-p34 | Spiky (SCAR-like)a | Yes | Yes | ARP2/3 complex | ARPC2 | ARPC2 | N/A |
| FBgn0038369 | Arpc3a | Spiky (SCAR-like)a | Yes | Yes | ARP2/3 complex | ARPC3A | No | N/A |
| FBgn0065032 | Arpc3b | Spiky (SCAR-like)a | Yes | Yes | ARP2/3 complex | ARPC3B | No | N/A |
| FBgn0001961 | Sop2 | Spiky (SCAR-like) | Yes | Yes | ARP2/3 complex | ARPC1A, ARPC1B | ARPC1A | N/A |
| FBgn0033260 | Cul-4 | Spiky (SCAR-like)b | No | Yes | ubiquitin-dependent protein degradation | CUL4A, CUL4B | Not screened | N/A |
| FBgn0025638 | Roc1a | Spiky (SCAR-like)b | No | Yes | ubiquitin-dependent protein degradation | RBX1 | RBX1 | N/A |
| FBgn0022213 | Cas | Nuclear actin | No | Yes | Nuclear transport | Cse1l | N/A | No |
| FBgn0030121 | CG17446 | Nuclear actin | No | Yes | Transcription | Cxxc1 | N/A | No |
| FBgn0031492 | CG3542 | Nuclear actin | No | No | Splicing | Prpf40a, Prpf40b | N/A | N/A |
| FBgn0037093 | CG7597 | Nuclear actin | No | Yes | Splicing (kinase) | Crkrs, Cdc2l5 | N/A | Cdc2l5 (Crkrs not tested) |
| FBgn0001337 | Exp6 | Nuclear actin | Yes | Yes | Nuclear transport | Xpo6 | N/A | Yes |
| FBgn0037657 | hyx | Nuclear actin | No | Yes | Transcription | Cdc73 | N/A | Yes |
| FBgn0016696 | Pitslre | Nuclear actin | No | No | Splicing (kinase) | Cdc2l1 | N/A | N/A |
| FBgn0003449 | snf | Nuclear actin | No | Yes | Splicing | Snrpa, Snrpb2 | N/A | Too toxic to assess |
a
Spiky (SCAR-like) annotation added after re-assessment with a longer RNAi period. See text for details.
b
Spiky (SCAR-like) annotation added after network analysis and reassessment. See text for details.