Table 7.
Yeast strains and oligonucleotides used in this study
| Strain
|
Genotype
|
Source
|
|---|---|---|
| W303-1B | MATα ade2-1 can 1-100 his3-11,15 leu2-3 trp1-1 ura3-1 | Alan Myers |
| AMR26 | W303-1B sir1::LEU2 | Rolf Sternglanz |
| YDS71 | W303-1B sir2::TRP1 | Rolf Sternglanz |
| YDS73 | W303-1B sir3::LEU2 | Rolf Sternglanz |
| YAB61 | W303-1B HMR-E aeb | Rolf Sternglanz |
| JRY4582 | W303-1B ADE2 Δlys2 sir4::LEU2 | Jasper Rine |
| YSZ269 | JRY4582 TRP1 sir4-42 | this study |
| YYZ1 | W303-1B rad52::TRP1 | this study |
| YYZ3 | YDS71 rad52::LEU2 | this study |
| YYZ4 | RS862 rad52::LEU2 | this study |
| YYZ5 | JRY4582 rad52::TRP1 | this study |
| YYZ48 | YSZ269 rad52::TRP1 | this study |
| YYZ212 | JRY4582 hmr::TRP1 | this study |
| YYZ213 | AMR26 hmr::TRP1 | this study |
| YYZ214 | YDS71 hmr::LEU2 | this study |
| YYZ215 | YDS73 hmr::TRP1 | this study |
| Oligo | Sequence | Gene |
| DVO208 | 5′-CAGCCGGAATGCTTGGCCA-3′ | HIS3 |
| DVO308 | 5′-AGATCTCACCATGGAAAGGCTTCG-3′ | SNF6 |
| DVO309 | 5′-ATCGATGAACGGCTCATTGGAATAA-3′ | SNF6 |
| DVO392 | 5′-ATCTCCAAGAGAGTTGGG | RAD52 |
| DVO393 | 5′-GCCTCAGCAGGTGCGGCC | RAD52 |
| DVO495 | 5′-CCATAGTTTCTGTGTACAAGAGT-3′ | Ty5 LTR |
| DVO496 | 5′-CTTGTCTAAAACATTACTGAAACAAT-3′ | Ty5 LTR |
| DVO583 | 5′-GGACAAATAGTGTATTCAGCCGTC-3′ | Ty5 GAG |
| DVO247 | 5′-CACGAGCTCATCTAGAGCC-3′ | HML-E |
| DVO212 | 5′-ACCAGAGAGTGTAACAACAG-3′ | HMR-E and HML-E |
| DVO251 | 5′-TGCTGAAGTACGTGGTGAC-3′ | HMR-I and HML-I |
| DVO252 | 5′-TTCTCGAAGTAAGCATCAAC-3′ | HML-I |
| DVO211 | 5′-TGGTAGAAGCAGTAGTAACT-3′ | HMR-E |
| DVO253 | 5′-AGCCCTATTCGCGTCGTG-3′ | HMR-I |
| DVO1066 | 5′-CCCGACTATGCTATTTTAATCATTGAAAACGAA TTTATTTAGATTGTACTGAGAGTGCAC-3′ | HMR |
| DVO1069 | 5′-TATCGTCATATACAAATCTAGAAATTACCAGAG CTATCCACTGTGCGGTATTTTCACACCG-3′ | HMR |
| DVO1070 | 5′-TACGAAGATTCTCGATTCCG | HMR |
| DVO1079 | 5′-TTACTGTTGCGGAAAGCTGA | HMR |
| DVO553 | 5′-CACCCACTACACTACTCGGTCAGG-3′ | rDNA |
| DVO554 | 5′-ATCTGGTTGATCCTGCCAGTAGTC-3′ | rDNA |
| DVO555 | 5′-TCTGTTTGGTAGTGAGTGATACTC-3′ | rDNA |
| DVO556 | 5′-AGACCCTGTTGAGCTTGACTCTAG-3′ | rDNA |
| DVO575 | 5′-TTCCGCACCTTTTCCTCTGTCCAC-3′ | rDNA |
| DVO576 | 5′-AAGACCCGAATGGGCCCTGTATCG-3′ | rDNA |
| DVO577 | 5′-AGAGGGCACAAAACACCATGTCTG-3′ | rDNA |
| DVO578 | 5′-TATCCGAATGAACTGTTCCTCTCG-3′ | rDNA |
| DVO408 | 5′-GGACTAGTTAAGTACCAGTTTGTGAATGT-3′ | sir4-42 |
| DVO409 | 5′CCCAAGCTTACAGAACAAGCCTGATGCA-3′ | SIR4 |